How to Cite
Abstract
Background: Optimization of nutrient feeding was developed to improve the growth of Bacillus subtilis in fed batch fermentation to increase the production of Jiean-peptide (JAA1). A central composite design (CCD) was used to obtain a model describing the relationship between glucose, total nitrogen, and the maximum cell dry weight in the culture broth with fed batch fermentation in a 5l fermentor.
Results: The results were analyzed using response surface methodology (RSM), and the optimized values of glucose and total nitrogen concentration were 30.70 g/l and 1.68 g/l in the culture, respectively. The highest cell dry weight was improved to 77.50 g/l in fed batch fermentation, which is 280% higher than the batch fermentation concentration (20.37 g/l). This led to a 44% increase of JAA production in fed batch fermentation as compared to the production of batch fermentation.
Conclusion: The results of this work improve the present production of JAA and may be adopted for other objective products' production.
Upon acceptance of an article by the journal, authors will be asked to transfer the copyright to Electronic Journal of Biotechnology, which is committed to maintain the electronic access to the journal and to administer a policy of fair control and ensure the widest possible dissemination of the information. The author can use the article for academic purposes, stating clearly the following: "Published in Electronic Journal of Biotechnology at DOI:10.2225/volXX-issueX-fulltext-XX".
The Copyright Transfer Agreement must be submitted as a signed scanned copy to biotec@ucv.cl. All authors must send a copy of this document.