Effect of cytokinin types, concentrations and their interactions on in vitro shoot regeneration of Chlorophytum borivilianum Sant. & Fernandez

Abstract

The present study describes an efficient and rapid technique for shoot multiplication and elongation of Chlorophytum borivilianum, an endangered medicinal plant. In this study, young shoot buds of C. borivilianum were cultured on MS medium containing 6-benzylaminopurine (BAP) and Kinetin (Kn), both at 0, 8.88, 17.8 and 26.6 µM, either individually or in combinations. Proliferated shoots were subcultured on fresh medium of the same constituents on week 3 of culture for further shoot multiplication and elongation. BAP alone (8.88 - 26.6 µM) was significantly effective on shoot multiplication, while Kn alone (8.88 - 26.6 µM) was significantly effective on shoot elongation compared to the control containing MS basal medium without any plant growth regulator. However, combination of both cytokinins stimulated an interaction producing higher shoot number and shoot length compared to their individual application. The most suitable combination was 8.88 µM BAP + 8.88 µM Kn, reaching a mean shoot number of 10.83 and shoot length of 6.85 cm.