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Sulieman AK, Dharma Putra M, Abasaeed AE, Gaily MH, Al-Zahrani SM, Zeinelabdeen MA. Kinetic modeling of the simultaneous production of ethanol and fructose by Saccharomyces cerevisiae. Electron. J. Biotechnol. [Internet]. 2018 Jul. 9 [cited 2024 Sep. 19];34. Available from: https://preprints.pucv.cl/index.php/ejbiotechnology/article/view/2018.04.006
Abstract
Background: Ethanol and fructose are two important industrial products that enjoy many uses. In this contribution, their production via selective fermentation of date extract using Saccharomyces cerevisiae was studied. Scaling up the process for possible commercialization was investigated in three fermentors with working volume ratio of 1:40:400.Results: Higher ethanol concentration was obtained in the larger fermentor due to conversion of fructose. Fructose yields in the 0.5-L, 7.5-L and 80-L fermentors were 99, 92 and 90%, respectively. Good fitting was obtained with the modified Monod kinetics; however, a better fit of cell mass was obtained with the modified Ghose-Tyagi model which accounts for ethanol inhibition.
Conclusions: The modified Gompertz model was expanded to facilitate prediction of products' formation and fructose fractions in all three fermentors. Such expansion will be beneficial in industrial applications.
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