Production of carotenoid sarcinaxanthin by Kocuria palustris isolated from Northeastern Brazil Caatinga soil and their antioxidant and photoprotective activities

Abstract

Background: This study aimed to produce carotenoids of two bacterial strains obtained and isolated from Caatinga soil in Northeastern Brazil and to evaluate their antioxidant and photoprotective activities. The morphological identification of bacteria was performed by Gram staining and molecularly confirmed through the 16S rRNA gene. The production of carotenoids was performed on two 2³ factorial designs to analyze the influence of independent variables (temperature range, luminosity, agitation, spiral presence, and bacterial isolate type) for maximum carotenoid yield. The selected condition has been transferred to a bioreactor (10L). The identification of carotenoids was performed by liquid chromatography (HPLC) and mass spectrometry (LC-MS). Antioxidant activity was determined by inhibiting the β-carotene/linoleic acid system and the effectiveness as sunscreen was measured through its sun protection factor (SPF).

Results: The results revealed that the isolates FT-7.22 and FT-5.12 were identified as Kocuria palustris; producers of a rare C50 carotenoid sarcinaxanthin. This is the first report on the production of carotenoids by this species from the Caatinga Domain. The pigment that was obtained from the Tryptic Soy Broth (TSB) medium in the best conditions of the factorial designs (increased agitation, aeration, and light exposure) exhibited a significant increase in the carotenoid production. The isolated FT-7.22 reached a higher sarcinaxanthin concentration (112,480 μg/L), and it exhibited promising antioxidant (76.53 ± 0.09%) and photoprotective activities (SPF = 9.36 ± 0.52).

Conclusions: This study demonstrated the ability of K. palustris to produce carotenoid sarcinaxanthin with antioxidant and photoprotective activities so that it can be applied in cosmetic formulations.