Genetic and physical map of broad host range cosmid pRG930cm
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Keywords

cosmid
pRG930cm
Pseudomonas
sequencing

How to Cite

1.
Chibeu A, Lavigne R, Mattheus W, Matthijs S, Cornelis P, Volckaert G. Genetic and physical map of broad host range cosmid pRG930cm. Electron. J. Biotechnol. [Internet]. 2009 Apr. 15 [cited 2024 Sep. 19];12(2):0-. Available from: https://preprints.pucv.cl/index.php/ejbiotechnology/article/view/v12n2-5

Abstract

We hereby present the complete sequence and annotation of pRG930cm, a spectinomycin/streptomycin/chloramphenicol-resistant cosmid vector. pRG930cm (17,256 bp; GenBank Accession No.: FM174471) has a broad host range, and is stably maintained by a number of Gram-negative bacteria including Pseudomonas spp, Escherichia coli, Agrobacterium tumefaciens and Azorhizobium caulinodans ORS571. pRG930cm is already widely used and its sequence will aid efficient construction and analysis of cosmid libraries.

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