Isolation of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene and expression analysis of anthocyanin biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.)
Vol. 15 No. 6 (2012), Short Communications
Vol. 15 No. 6 (2012)

Isolation of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene and expression analysis of anthocyanin biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.)

Short Communications
Published 2012-10-23
Da Qiu Zhao
Yangzhou University
Chen Xia Han
Yangzhou University
Jin Tao Ge
Yangzhou University
Jun Tao
Yangzhou University
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Keywords

anthocyanin
expression
glucosyltransferase
herbaceous peony.

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1.
Zhao DQ, Han CX, Ge JT, Tao J. Isolation of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene and expression analysis of anthocyanin biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.). Electron. J. Biotechnol. [Internet]. 2012 Oct. 23 [cited 2024 Sep. 19];15(6). Available from: https://preprints.pucv.cl/index.php/ejbiotechnology/article/view/v15n6-5
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Abstract

Herbaceous peony (Paeonia lactiflora Pall.) is an excellent material for studying the formation of flower colour because of its abundant colour. The full-length cDNA of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene (UF5GT) containing 1629 bp nucleotides was obtained from P. lactiflora. The expression patterns of nine related anthocyanin biosynthetic genes (PlPSY, PlCHS, PlCHI, PlF3H, PlF3'H, PlDFR, PlANS, PlUF3GT and PlUF5GT) in diurnal variation petals showed that their expression peaks were basically at 15:00 and the expression patterns were consistent with the trend of sampling conditions except individual gene. And the highest expression levels were in PlCHS, PlDFR and PlUF3GT, which could be the candidates to regulate P. lactiflora flower colour by means of genetic engineering.
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