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How to Cite
1.
Kuhn J, Müller H, Salzig D, Czermak P. A rapid method for an offline glycerol determination during microbial fermentation. Electron. J. Biotechnol. [Internet]. 2015 May 27 [cited 2024 Sep. 19];18(3). Available from: https://preprints.pucv.cl/index.php/ejbiotechnology/article/view/2015.01.005
Abstract
The purpose of this work was to find a rapid method for glycerol detection during microbial fermentations. The method requirements were, first, to avoid sample pretreatment, and second, to measure glycerol precisely especially out of fermentation broth. This was achieved by combining two reaction principles - the malaprade reaction and the hantzsch reaction. In the malaprade reaction, glycerol is converted into formaldehyde. This forms a dye in the hantzsch reaction after which adsorption is than detected. The subsequent assay was investigated with two different fermentation media, a chemically undefined and a chemically defined media, used for Pichia pastoris fermentation. In both media, as well as in real fermentation samples, glycerol content could be reproducibly detected with the method. Moreover, measurements were more precise than using a standard glycerol detection kit.Upon acceptance of an article by the journal, authors will be asked to transfer the copyright to Electronic Journal of Biotechnology, which is committed to maintain the electronic access to the journal and to administer a policy of fair control and ensure the widest possible dissemination of the information. The author can use the article for academic purposes, stating clearly the following: "Published in Electronic Journal of Biotechnology at DOI:10.2225/volXX-issueX-fulltext-XX".
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